GENETIC CHARACTERISTICS OF ACTINOMYCETES ISOLATES (GST, KP, KP11, KP16, T24, T37 CODE) BY RFLP OF NRPS GENES
ABSTRACT: Actinomycetes is the
one of antibiotic producing microorganisms. Actinomycetes isolates with GST,
KP, KP11, KP16, T24, T37 have been isolated from soil. This study aims to
determine the diversity of actinomycetes
isolates by RFLP (Restriction Fragment Length Polymorphism) of NRPS (Non Ribosomal Peptide Synthetase)
genes from GST, KP, KP11, KP16, T24, and T37 isolates. This study is divided
into several steps of the DNA isolation
from isolate GST, KP16, T24, T37, KP, KP11, PCR of the 16S rRNA and NRPS
genes and cutting the NRPS genes fragmens with HaeIII enzyme to determine the
diversity of isolates. Results of DNA isolate, PCR of 16S rRNA genes and NRPS
genes, and RFLP of NRPS genes performed by agarose gel electrophoresis. The
diversity of the isolates were analyzed by multivariate analysis UPGMA with
MVSP 2.0 applications. The results
showed the diversity of 6 actinomycetes isolates are T37 and GST have 64 %
similarity, KP11 and KP16 have 96 % similarity, KP has 76 % similarity with KP11
and KP16, while T24 has 60 % similarity with KP and 12 % similarity with T37
and GST. Based on RFLP analysis of the NRPS genes, among of 6 isolates can
divided into 4 groups are T37 and GST isolates as group 1, KP11 and KP16 isolates
as group 2, KP isolate as group 3, and T24 isolate as group 4.
Author: Melati Aprilliana
Ramadhani, Nanik Sulistyani
Journal Code: jpfarmasigg150024
