Dexamethasone Activities toward Population of B cells, Gr-1, and TNF-α cytokine in Mice (Musmusculus) Balb/c Biliary Atresia Model
ABSTRACT: Biliary atresia
iscondition caused by
Rotavirus (RRV) infection. The
aims of this
study were to
know the immune responses
of mice model
of biliary atresia
treated with corticosteroid.Mice were
splitinto 3 treatment groups: control
(K), RRV injection (R), and RRV injection in the present of dexamethasone(R+D).
In R
treatment, the baby
mice born in
<24 hours were
injected with 20
µl of phosphate
buffered saline containing 1.5 x
106 fluorescence-forming units Rhesus Rotavirus (RRV). First termination was
performed in the day 7 to 14, while second termination was done in the day 14
to 21. The dosage of dexamethasone which is applied in
this experiment is
0.5mg/kg body weight.Immunocompetent cells
were isolated from
spleen, and cell surface
molecules were then analyzed by
flowcytometry. The data was tested by SPSS 16.0 for Windows program. The
results showed that
dexamethasone given as
corticosteroid for biliarry
atresia theurapy couldsuppress
TNF-αproduction as well as Gr-1 proliferation. In the other hand dexamethasonecan promote B220+ cell
proliferation inrotavirus infected mice.
Author: Riza Rahmawati,
Muhaimin Rifa’I
Journal Code: jpbiologigg140004
