Activity of Dexamethasone Therapy on Pro-Inflammatory Cytokines Profile of Balb/c Mice with Biliary Atresia
ABSTRACT: Biliary atresia
is a neonatal
obstructive cholangiopathy that
progresses to end-stage
liver disease. Dexamethasone is
one of synthetic glucocorticoid which has function as an anti-inflammatory
agent. Here, we investigated
whether dexamethasone could
modulate the immune
activity in mice
strain Balb/c with
biliary atresia based on
the change of
quantity of IFN-γ
and TNF-α as
a pro-inflammatory molecules.
This study consists of 2 stages.
The first stage is pre-condition which is made the biliary duct become fibrosis
by injecting 20 µl of
phosphate buffered saline
containing 1.5 x
106 fluorescence-forming units
Rhesus Rotavirus (RRV) subcutaneously on
first day (24
hours) after the
mice was born.
The second stage
is injection with dexamethasone with
dose 0,5 mg/kg
BW subcutaneously on
the 7th-14th day
and 14th-21st day.
The clinical effect of
dexamethasone is
investigated on 14th
and 21st day
by flow cytometry
method. Data were analyzed using Kruskal-Wallis test
(p<0,05) and Mann-Whitney test using SPSS 16 for Windows. Rotavirus injection subcutaneously
was proven to stimulate the production of proinflammatory cytokines, especially
in the third week of termination. The result indicated an increasing number of
proinflammatory cytokines such as IFN-γ and
TNF-α after RRV
injection but after
injection of dexamethasone
the number of
those cytokines is decreased.
It can be
understood that dexamethasone
has a capability
to reduce the
effect of inflammation regard to the decrease of
proinflammatory cytokines.
Author: Qonitatul Khasanah,
Muhamin Rifa’i
Journal Code: jpbiologigg140003
