Bioactivity of Proplis to the CD4 + and CD8+ T cells Producing IFN-γ Cytokines in BALB / C Mice
ABSTRACT: Propolis (bee glue)
is a natural resinous product of honey bees which collected from exudates and
plant buds, rich in
biochemicals constituents including
mostly flavonoids, phenols
and various acids
bond. These compound are
believed to be
responsible as immunomodulatory agents.
The study aims
to determine the immunomodulatory activity
of ethanolic extract
of propolis to
the CD4+ and
CD8+ T cells
producing IFN-γ cytokines and
analyze the differences
immune responses between
control and treatment
group by in
vivo. Stages include animal
acclimation for ±
1 week, preparation
of Ethanolic Extracts
of Propolis / EEP, Oral Administration with doses levels of 0 mg
/ kg BW; 50 mg / kg BW (DI); 100 mg / kg BW( DII); 200 mg / kg BW (DIII) for 2 weeks, isolation
of lymphocyte cells from spleen, flowcytometry analysis to asses cell number and
surface molecule expression. Data was analyzed using Kruskal Wallis Test with α
= 0,05 and followed by Mann Whitney Test by SPSS 16.0 for windows with complete
randomized design. The results showed that a dose of
50 mg /
kg BW was
increases the relative
number of CD8+
T cells producing
IFN-γ cytokines significantly (p
<0.05) compared with controls. However, at the same dose the relative number
of CD4+ T cells producing IFN-γ cytokines
was decreased significantly (p<0,05). Based
on this case,
its dose supposedly that the
ethanolic extract of
propolis play role
in maintaining the
balance or homeostatic
of IFN-γ cytokines production by T cell subsets. Dose
of 100 mg/kg BW and 200 mg / kg BW could decrease the relative number of
activated CD4+ T cells producing IFN-γ cytokine significantly compared to
controls.
Author: Yonna Ayundria,
Muhaimin Rifa’I
Journal Code: jpbiologigg140005
