Uji Penghambatan Aktivitas alfa-glukosidase Ekstrak dan Fraksi Daun Antidesma neurocarpum Miq.
Abstract: Diabetes mellitus is
a metabolic dysfunction disease showed by hyperglicemia and abnormality of
carbohydrates, fats, and proteins metabolism. It is known can be cured with
a-glucosidase inhibitor. Previous experiment reported that methanol extract
from leaves of Antidesma neurocarpum Miq. has a strong inhibitory activity of
a-glucosidase (IC50 = 2,18 mikrogram/mL). The aim of this experiment was to
know about the most active extract and fraction of inhibitory activity of
a-glycosidase and also the chemical compounds from those most active extract
and fraction. Extraction is done by using multilevel maceration (n-hexane,
ethyl acetate and methanol). Inhibitory activity of a-glucosidase tested with microplate
reader at 405 nm wavelength. As standard, we used carbose (IC50=38.37 gg/mL).
Methanol extract has the strongest inhibitory activity of a-glucosidase as
shown the highest inhibitor percentage (89.53%). It is identified the chemical
compounds and fracinationed by flash column chromatography. Fractination produced
15 combined fractions. Combined fractions which more than 200 mg tested to know
their inhibitory activity of a-glucosidase. Result showed that the most active
fraction is the 8th (IC50=40.77 gg/mL) with a competitive inhibitor mechanism
to a-glycosidase. Chemical compounds that is found in the methanol extract and
8th fraction of Antidesma neurocarpum Miq. leaves are flavonoids, tannins,
glycosides, fenol and alkaloids.
Penulis: Rieka Widihasputri
Kode Jurnal: jpfarmasidd120138
