Efek Antidiabetes dan Identifikasi Senyawa Dominan Fraksi Kloroform Herba Ciplukan (Physalis angulata L.)
Abstract: Antidiabetic
activity of ciplukan (Physalis angulata L.) herb has been widely observed, as
an extracts and infusions, proven to reduce blood sugar levels in rats or mice
induced by alloxan. The research objective was to evaluate the antidiabetic
effect and identify the content of the chloroform fraction ciplukan herb
eluated with methanol-ammonia. An- tidiabetic effects testing conducted using
30 male white mice (Mus musculus) ddY strain were divided into 6 groups, each
consisting of 5 mice. K-1 is a negative control induced by alloxan, K-2, K-3
and K-4 induced by alloxan and treated with the test substance dose of 50 mg/kg
bw/day, 100 mg/ kg bw/day, 200 mg/kg bw/day, K-5 is a positive control, induced
by alloxan and treated with metformin dose of 65 mg/kg bw, K-6 as normal
controls . Each mouse blood samples taken on day 7 and day 14, to test their
blood sugar levels. Data were analyzed using one-way anova and multiple
differences test. Identification of the content of the chloroform fraction
eluated with methanol-ammonia, carried out by gas chroma- tography mass
spectrometry (GC-MS). The results showed that all three doses showed a decrease
in blood sugar levels significantly at day 7 , but the decline has not reach
normal levels. Blood sugar levels drop significantly and the decline has
reached normal occurred on day 14. Dominant content of the chloroform fraction
eluated with methanol-ammonia is a class of unsaturated fatty acid chain length
is Hexanoic acid, C16H1202, hexadecanoic acid methyl ester, C17H3402,
9-octadecenoic acid methyl ester, C19H3602, Oleic acid butyl ester, C22H4202,
9-octadecenoic acid, C19H3602, Octadecanoic acid, C18H3602, 1,2-Benzendicarboxylic
acid, C8H604, and Aplysterylacetate, C31H5202. Another result is that
Nordextromethorphan alkaloid compound, Cl 7H23NO. The conclusion is chloroform
fraction of ciplukan herb has antidiabetic effects and has a class of compounds
content of unsaturated fatty acids, Aplysterylacetate and alkaloid
Nordextromethorphan.
Penulis: Sediarso
Kode Jurnal: jpfarmasidd120139
