ISOLASI SENYAWA ANTIMIKROBA DARI SPON LAUT Pseudoceratina purpurea CARTER
ABSTRACT: An antibacterial
compound (HC 2) has been isolated from the n-hexane fraction of marine sponge Pseudoceratina purpurea
Carter. Isolation of
HC 2 compound
was done by
chromatographic method
followed by recrystalization. The
antimicrobial assay was
guided by diffusion
method. The compound HC 2 was white needle crystals with melting point
125-127oC. HC 2 compound was tested for an antibacterial activity
at concentrations of 5%, 3% and
1% against Escherichia coli, Pseudomonas
aeruginosa, Staphylococcus aureus
dan Staphylococcus epidermidis bacterials, respectively.
Results showed that
HC 2 compound
had low activity
against all bacteria. Identification and characterization of the
active compound was confirmed by chemical reactions and infrared spectrum data.
HC 2 compound was identified as steroid.
Penulis: Dian Handayani,
Martha Sririta, M. Husni Mukhtar
Kode Jurnal: jpfarmasidd100039
