FORMULASI MIKROENKAPSULASI PROTEIN DALAM POLI (D,L-LAKTIDA) DENGAN TEKNIK PENGUAPAN PELARUT
ABSTRACT: Poly (D,L-lactide
acid) has been
used as scaffold
and controlled release
device for protein such
as growth factor
in tissue engineering.
In this study,
PDLLA microparticles were
made and papain was
used as model
protein. Protein was
encapsulated in microparticles using water-oil-water (W1/O/W2)
and solid-oil-water (S/O/W)
emulsification-solvent
evaporation. Types of encapsulation methods and ratios of papain-PEG
20000 were observed in this study to provide the highest encapsulation
efficiency. The entrapment efficiency made by W1/O/W2 method was
6,38%0,025, whilst S/O/W
using ratios of
papain-PEG 20000 1:1
; 1:4 ; and 1:5
were 6,24%0,91 ; 30,15%1,66 and 60,67%4,93, respectively. To conclude, S/O/W
is the best method to encapsulate protein with highest entrapment efficiency.
Penulis: Lili Fitriani, Heni
Rachmawati, Tri Suciati
Kode Jurnal: jpfarmasidd100040
