PRODUKSI BIOETANOL DARI AMPAS SAGU (Metroxylon sp) MELALUI PROSES PRETREATMENT DAN METODE SIMULTANEOUS SACCHARIFICATION FERMENTATION (SSF)
Abstract: Sago waste is one of
biomass lignocellulose that potential to produce bioethanol. Lignocellulose
material consist of three major component such as cellulose, hemicellulose and
lignin. The available of lignin become inhibitor for enzymatic process it must
removed by pretreatment. Research for bioethanol production from sago waste
through pretreatment process by using a variation of alkaline solution; 1%
NaOH, 8% NH4OH, 1% NaOH + 4% NH4OH and 1% NaOH + 8% NH4OH with a ratio solid to liquid 1:10 (w/v) after
that, followed by SSF method involved two kind of fungi like Trichoderma viride
strain T1 sk for saccharification and Saccharomyces cerevisiae for fermentation
process. The optimum conditions for the reduction of samples obtained after
pretreatment at concentration of 1% NaOH + 8% NH4OH at 49,3017% with a long
incubation period of 3 days with a temperature 500C. Result of determination
cellulase enzyme activity from
Trichoderma viride strain T1 sk by 0.1% CMC is 0,1144 unit and the highest
glucose yield is 921,25 µg/mL from 0,9 gram substrate of sago waste while the
optimum time for the saccharification process obtained in 75 minutes. Ethanol
content was analyzed by GC/MS resulted 12.99% (% area) or equal with 0,38 mL
ethanol yield for 168 hours of fermentation .
Penulis: Khairunnisah,
Marniati Salim, dan Elida Mardiah
Kode Jurnal: jpkimiadd130200
