PENGUJIAN EKSTRAK ASETON DAUN BAYAM (Amaranthus sp) SEBAGAI SENYAWA ANTIRADIKAL DPPH, ANTIBAKTERI DAN IDENTIFIKASI SENYAWA AKTIF DENGAN KG SM
Abstract: This research
reports antiradical and antibacterial activities of three of spinach
(amaranthus sp) leaves acetone extract were red spinach (Amaranthus tricolor L.
) , green spinach ( Amaranthus hybridus L.) and spines spinach ( Amaranthus
spinosus L ). Fresh spinach leaves were dried and extracted by maceration with
acetone for 24 hours. The extracts tested antiradical DPPH activity by
spectrophotometry at a wavelength of 517 nm. The results showed that acetone
extract of three spinach types have DPPH scavenging activity by red spinach
leaves (IC50 = 29.76 ug/ml), green spinach leaves (IC50 = 46,55ug/ml) and spines spinach (IC50 =73.66 ug / ml)
extract. Antibacterial activity was tested against the bacteria Staphylococcus
aureus and Escherichia coli by agar diffusion method. The results showed that
the acetone extracts of leaves of green spinach and spines spinach inhibited
Staphylococcus aureus respectively, diammeter of clear zone 3.00 mm and 3.21
mm, the results no inhibition to the growth of Escherichia coli. Acetone
extract of red spinach not shown clear zone against the growth of both
bacteria. Identification of active compound by GCMS contained the fatty acids
and tocopherols .
Penulis: Kusmiati
Kode Jurnal: jpbiologidd140131
