UJI AKTIVITAS ANTIMIKROBA DAN TOKSISITAS DENGAN METODE BSLT SERTA PENAPISAN FITOKIMIA EKSTRAK DAUN ALAMANDA (Allamanda cathartica L.)
Abstract: Alamanda (Allamanda
cathartica L.) leaves has an antioxidant and toxicitypotential. Leaves
extracted by maceration with ethanol, and partitioned with petroleum ether ,
ethyl acetate , methanol and chloroform. The extract was tested the
antimicrobial activity using agar diffusion method and its toxicity test by
Brine Shrimp Lethality Test (BSLT)method.Phytochemical screening of the extract
condensed done to determine the content of secondary metabolites. The
Antimicrobial activity test showed that the phase of petroleum ether , ethyl
acetate, chloroform and metanol alamanda leaves potentially inhibit Bacillus
subtilis, Micrococcus luteus, Candida albicans and Saccharomyces cerevisiae.
The highest antimicrobial activity was observed
for the phase petroleum ether of alamanda which inhibited zone 10.45 mm
against Saccharomyces cerevisiae. Whereas the phase ethyl acetate, chloroform
and methanolphase of alamanda showed the largest inhibition zone respectively
7.33 mm , 8.59 mm and 7.02 mm against Candida albicans. The toxicity tests of
alamanda leaf extracts against larvae shrimp ( Artemia salina Leach ) showed
that the petroleum ether phase has LC50 values of 64.13 ppm was toxic , the
ethyl acetate phase at 26.55 ppm was highly toxic , the chloroform phase at
23.39 ppm was highly toxic and metanol extracts of 38.19 ppm were toxic .
Phytochemical screening of the leaf powder and methanol phase of alamanda
contained alkaloids , flavonoids , saponins , tannins and steroids /
triterpenoids . Petroleum ether phase contained steroids / triterpenoids ,
whereas the ethyl acetate phase contained alkaloids , saponins , tannins and
steroids , and the chloroform phase containing alkaloids , flavonoids , saponins
and steroids / triterpenoids .
Penulis: Kusmiati
Kode Jurnal: jpbiologidd140130
