PENAMBAHAN 6-BENZIL AMINO PURINE (BAP) DAN ASAM NAFTALENASETAT (NAA) BERHASIL MENGINDUKSI PEMBENTUKAN DAN PEMANJANGAN TUNAS MERBAU (INTSIA BIJUGA (COLEBR.) O. KUNTZE) SECARA IN VITRO
Abstract; Merbau(Intsia
bijuga(Colebr.) O.Kuntze) is one of the most valuable wooden tree in Indonesia
because of the high price and the international highly demanding on it continue
to rise up every year.On the other hand,due to an excessive exploitation and
unsuccesful reforestation programs resulted on a status of a vulnerable species
of the tree. The seedling production of the species using traditional technique
is still limited to overcome the problems because of the low number of the seed
production every year. One of the most posible approch for mass rapid plantlets
production of merbau is using shoot tip culture. However, the protocol of
merbau shoot tip culture is still not well developed since the success rate of
shoot induction remains low (less than 15 %), no multiplication, root induction
and acclimatisation step are aplicable. Our results indicate that DKW
medium(Driver and Kuniyuki, 1984) suplemented with 10-6 MNAA and 10-6 MBA
Pinduce shoots formation fromaxillary budex plant with a success rate up to
80%. The number of shoots produced from each explant was two shoots with six
internodes and quite tall (4,5 cm). The multiplication can be done by the
sub-culture the axilary buds into fresh medium every four weeks. The next step
will be induction and acclimatisation of the plant podused by shoot tip
culture.
Penulis: Kiki Ernawati
Kode Jurnal: jpbiologidd140125
