KEMAJUAN PENELITIAN INDUKSI DAN PEMANJANGAN TUNAS MERBAU (INTSIA BIJUGA [COLEBR.] O. KUNTZE) SECARA IN VITRO
Abstract: Merbau is one of the
high-quality timber tree with the demanding of international market increase
every year. However, the over-exploitation of the tree put merbau as a
vulnerable species in the future years. Moreover, seedling production of merbau
using traditional technique showed several limitations due to the low number of seed production
every year. One of the most promising technique for mass seedling production of
merbau is using shoot-tip culture. However, the protocol of shoot-tip culture
is remain under develop with low success rate of shoot induction (less then 15
%), without protocols on shoot multiplication, root induction and
acclimatisation. Our research at the Laboratory of Botany and Genetics,
University of Muhammadiyah Purwokerto showed that the axillary buds isolated
from 1-month seedlings can be maintained to induce shoot formation and
elongation on the DKW medium (Driver and Kuniyuki, 1984) supplemented with 10-8
M kinetin. Two new shoots started to elongate from each explant after 4 weeks
culture (60 % success rate) with each shoot has the lenght of c.a. 4 cm and 4
internode. The inducted shoots were then cut them of into four new axilary buds
and subcultured onto the same medium every two week for shoots multiplication.
The next step of shoot-tip culture technique, root induction and
acclimatization stages is remain developed.
Penulis: Latifah Esti
Ramadhani
Kode Jurnal: jpbiologidd140126
