Pengaruh Xantorizol terhadap Sel Hepatoma HepG2
Abstract: The investigation of
xanthorrizol antiproliferative effect and the mechanism of cell death in human
hepatoma cell line HepG2 had been investigated. Antiproliferative assay using
methylene blue staining revealed that xantorizol inhibited the proliferation of
HepG2 cells with an IC50 value of 4.17 ± 0.03 μg/ml. The antiproliferative
activity of xanthorrhizol was due to apoptosis induced in HepG2 cells and not
necrosis, which was confirmed by the Tdt-mediated dUTP nick end labeling
(TUNEL) assay. The xanthorrhizol-treated HepG2 cells showed typical apoptotic
morphology such as DNA fragmentation. The apoptosis mediated by xanthorrhizol
in HepG2 cells was associated with the activation of tumor suppressor p53 and
down-regulation of the antiapoptotic Bcl-2 protein expression, but not Bax. The
levels of Bcl-2 protein expression decreased 24-hrs after treatment with
xanthorrhizol, and remained lower than controls throughout the experiment
resulting in a shift in the Bax to Bcl-2 ratio thus favouring apoptosis. In
conclusion, xanthorrhizol exerts antiproliverative effects on HepG2 cells via
apoptosis by modulating p53 and Bcl-2 protein levels.
Penulis: Tri Handayani
Kode Jurnal: jpkedokterandd080028
