Deteksi Mutasi pada Quinolone Resistant Determining Regions (QRDRs ) Gen gyrA pada Salmonella typhi Isolat Klinik dan Galur Khas Indonesia
Abstract: A number
of researchers in
endemic regions in
Asia have proved
that mutations in
Quinolone Resistant
Determining Regions (QRDRs),
parts of gyrA
gene of Salmonella
typhi, can reduce
the susceptibility of ciprofloxacin therapeutic in enteric fever. The
sites of amino acid mutations in QRDRs in relation with
decreased fluoroquinolone susceptibility are
different in each
endemic region. The
present study attempted to
investigate the association
of the decrease of
fluoroquinolone
susceptibility and the mutations
in QRDRs of
clinical and typical
Indonesian Salmonella typhi
isolates. All isolates
were subjected to the antimicrobial susceptibility test applying the
Kirby-Bauer method of disk diffusion with ciprofloxacin and
were examined by PCR and
direct nucleotide sequencing
for genetic alteration
in QRDRs. One ciprofloxacin
sensitive isolate was
observed and it
showed no mutation;
ciprofloxacin resistant
galurs were observed
in 2 isolates
and they also
showed no mutation.
The data imply
that QRDRs mutations in
this research are
not associated with
fluoroquinolone resistance in
clinical and typical Indonesian
Salmonella typhi isolates. It is suggested that nucleotide re-sequencing should
be used to verify this result.
Penulis: Kirby Saputra, Daniel
Chandra, Johan Lucianus, Ernawati A. Giri Rachman
Kode Jurnal: jpkedokterandd090092
