Bakteri Coliform dalam Es Batu pada Tiga Rumah Makan Ayam Goreng Siap Saji di Bandung

Abstract: Some reports have shown that ice cubes may contain Enterobacteriaceae bacteria. This descriptive, observational research aims to analyze the microbiological quality of the ice cubes served at three selected restaurants  (1,2,3).  Three  samples  of  ice  cubes  were  rinsed  with  steril  water,  left  melted,  and  diluted serially.  Each  diluted  sample  was  inoculated  into  MacConkey  Agar  and  EMB  Agar  with  pour  plate method  duplo.  The data collected were the number of colonies (interpreted as cfu or colony forming  unit) of Escherichia coli and other Enterobacteriaceae bacteria. The species of selected colonies were identified by biochemical reactions. The result showed that sample 1 contained 3 cfu/ml Escherichia coli and 5 cfu/ml Pseudomonas  sp.  Sample  2  contained  10  cfu/ml  Escherichia  coli  and  4  cfu/ml  Pseudomonas  sp.,  but sample 3 didn’t contain Escherichia coli and other Enterobacteriaceae bacteria members. It is concluded that ice cubes from restaurant 3 met the requirement for drinking water, whereas those from  restaurants 1 and 2 did not.
Keywords: ice cubes, Escherichia coli, Pseudomonas sp
Penulis: Michael, Philips Onggowidjaja, Djaja Rusmana
Kode Jurnal: jpkedokterandd100055

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