Analisis DNA Bacillus sp. BAC4 Hasil Polymerase Chain Reaction (PCR) dengan Primer-Primer yang Dirancang dari Gen pga
Abstract: Bacillus sp. BAC4
DNA was amplified using PCR method employing primers designed from pga genes of
5 bacteria (Arthrobacter viscosus, Bacillus megaterium, Escherichia coli,
Kluyvera citrophila, and Providentia rettgeri). Analysis of DNA base direct sequencing of PCR products
showed the highest homology of Bacillus sp. BAC4 DNA with Bacillus subtilis DNA
at the region from citG to yirG. This
fact states that primers designed from pga genes of 5 bacteria has not been
successful in amplifying Bacillus sp. BAC4 pga gene, and that Bacillus sp. BAC4 pga gene is predicted to be quite
different from the existing data of pga genes available at Genbank. Analysis of DNA base direct sequencing
amplified using the pga primers shows the highest homology to the DNA of Bacillus
subtilis.
Penulis: Philips Onggowidjaja,
Maelita Ramdani Moeis, Enny Ratnaningsih
Kode Jurnal: jpkedokterandd080024
