Isolasi dan Karakterisasi Bakteri Asam Laktat pada Usus Ayam Broiler
ABSTRACT: The objective of the
research was to evaluate and characterize of a Lactic Acid Bacteria isolated
frombroiler’s small intestine. The research was done by three steps : (1)
Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation
and (3) Inhibitory test with pathogen bacteria. The isolation of lactic acid
bacteria was proceed by clone from broiler’s small intestine with MRS Broth for
24 hours, thinned until 10 ̄ 9with serial number, invested in MRS jell, purify
by streak plate method and storage the lactic acid bacteria in an MRS angle
jell. Gram colourize was done with crystal violet liquid, Iodium, Alkohol 95%
and Safranin, and also microscopic
observation was done by electron microscope with 40 zooming. Inhibitory test
with pathogen bacteria was evaluated with
Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 dan 48
hours incubation period.
The isolation result showed two sample of isolate (A1 dan A2) and consist
of the observation in shape as well as colour of the bacteria growth, for
sample of isolate A1 the colonies had coccus
and white colour. The isolate of A2 had sarcinar. After that, gram
colouring was done and microscopic observation were identified into
gram-negative bacteria became bacill and bacill cocco. Inhibitory test with
pathogen bacteria showed that it produced antimicrobial with 11 mm clearing
zone diameter. The result of the research got Lactobacillus sp as the genera of
Lactic Acid Bacteria.
Keywords: Lactic Acid
Bacteria; small intestine; broiler
Penulis: Meisji Liana Sari,
Arfan Abrar, Merint Merint
Kode Jurnal: jppeternakandd130018
