Abstract: Phytochemical test
and Antibacterial Activity test in each fraction from the gourds fruit
(Lagenari siceraria (Molina) Standl) that came from Teluk Dalam, Tenggarong
Seberang have been done. The sample of leaves was extracted with ethanol and
then consentrated by using rotary evaporator. The ethanol crude extract were
fractioned with n-hexane, Ethyl Acetat and Methanol. Based on the phytochemical
test on secondary metabolits of the gourds fruit (Lagenari siceraria (Molina) Standl),
it showed that ethanol crude extract contained flavonoid, saponin, steroid and
phenol. n-hexane fraction contained steroid, ethyl acetat fraction contained
phenol. And methanol-water fraction contained saponin, and phenol. Based on the
Antibacterial Activity test using the paper disc diffusion method (diameter 6
mm), the results of this test showed that the most active fraction is n-Heksana
with : of the Bacillus cereus bacteria at the concentration 1, 2, 4, 6, 8 and
10% (b/v) with inhibition diameter 9.57, 9.88, 10.17, 12.18, 13 and 14.3 mm
respectively so minimum inhibitory concentration got 0-1%. The active respons
toward Salmonella typhi bacteria at the concentration 1, 2, 4, 6, 8 and 10%
(b/v) with inhibition diameter 7.50, 7.60, 8.20, 8.38, 8.87, and 9.8 mm so minimum
inhibitory concentration got 0-1%. The chloramfenikol antibiotics concentration
0.00386 M as a standar to inhibit the growth of Bacillus cereus and Salmonella
typhi bacteria with the inhibition diameter 12.58 mm and 15.06 mm respectively.
Keywords: Lagenari siceraria
(Molina) Standl, phytochemical, and antibacterial activity
Penulis: Eva Marliana, Chairul
Saleh
Kode Jurnal: jpkimiadd100050
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