Abstract: The research about
optimation of analytical performance for determination of caffeine by using High
Performance Liquid Chromatography (HPLC) method has been done. The optimal of result
determination to obtain, the HPLC system has been optimalized for the important
parameters are composition of mobile phase, flow rate and eluen pH. The result
of research shown that the optimum conditions to obtain from the research are
composition of mobile phase methanol: water (70: 30), flow rate 0.5 mL / minute
and eluent pH are 5 has been achieved. Based on the optimalized condition, the
analytical performance of this method has been performed for the determination
of caffeine with HPLC method. The result of research shown reproducibility of
this method as coefficient of variation percentage (% CV) was 1.8665 %, limit
of detection was 33.1332 ppb. This result showed this method capable to
application for the routine analysis of caffeine in samples.
Keywords: Caffeine, HPLC,
analytical performance
Penulis: Aman Sentosa
Panggabean, Subur P. Pasaribu, Nisma Vinanda, Rita Hairani
Kode Jurnal: jpkimiadd100051
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