THE EFFECT OF VITAMIN C (L-ASCORBYL MONOPHOSPHATE-Mg) ON THE DEFORMITY PERFORMANCE OF HUMPBACK GROUPER (Cromileptes altivelis) LARVAE
Abstract: This study was aimed
to get a standard protocol for vitamin C application in enrichment of Artemia
nauplii and to examine the impact of vitamin C on humpback grouper larval
deformity performance. Vitamin C in the form of L-ascorbyl-2-monophosphate-Mg
(AMP-Mg) was used for the enrichment and incorporated into Artemia nauplii at a
given level of 0-1.5 g/L with enrichment period of 4, 6, and 24 hours for each
level. The effect of AMP on larval deformity was tested using the following
treatments: unenriched both Artemia nauplii and commercial diet, un-enriched
Artemia nauplii and experimental diet containing 2.5 g AMP-Mg/kg diet, enriched
Artemia nauplii obtained from the protocol and commercial larval diet. Analysis
of vitamin C content in Artemia nauplii was carried out using HPLC and larval
deformities were observed using staining method, visual examination, or X-ray
depending on larval age. The results showed that the best enrichment method of
AMP in Artemia nauplii is achieved at the dose of 0.9 g/L for 6 h. The lowest
percentage of abnormalities was found in larvae fed with enriched Artemia
nauplii and commercial diets, suggesting that vitamin C has an important role
in controlling larval deformities.
Keywords: humpback grouper larvae;
vitamin C; deformity
Author: Sophia Lasma Sagala,
Suko Ismi, I Nyoman Adiasmara Giri
Journal Code: jpperikanangg100012
