NONSPECIFIC IMMUNE RESPONSE AND RESISTANCE OF Litopenaeus vannamei FED WITH NUCLEOTIDE, β-GLUCAN, AND PROTAGEN DIETS
Abstract: The objective of
this research was to evaluate the nonspecific immune response and resistance of
Litopenaeus vannamei fed with nucleotide, β–glucan, and protagen diets. Shrimp
juveniles with an average weight of 5.39±0.56 g were reared in glass aquaria at
a density of 15 shrimps/aquarium. Shrimps were fed three times a day for four
weeks at a feeding rate of 3%/bw/day. Treatment diets consisted of A: basal
diet (without immunostimulant), B: β–glucan, C: protagen, and D: nucleotide,
each with three replicates. At the end of feeding period, the shrimps were
intramuscularly injected with Vibrio harveyi 0.1 x 106 cfu.shrimp-1. Total
haemocyte count (THC) of shrimp fed with nucleotide-diet was significantly
different compared to that of control shrimp (p=0.01), but not different
compared to shrimp fed with protagen-diet. PO activity also increased
significantly in shrimp fed with nucleotide-diet (p=0.02). β–glucan diet could
also increase THC and PO activity, but compared to the control, the increase was
not significantly different. Overall, PO activity of shrimp fed with
nucleotide, β–glucan, and protagen diets was high (>0.35). Oral
administration of nucleotide, β–glucan, and protagen for four consecutive weeks
significantly increased resistance of shrimp to disease (<0.01) where the
highest resistance rate was observed on shrimp fed with nucleotide-diet. Growth
of shrimp fed with nucleotide-diet was significantly different compared to that
of control shrimp (p<0.01), as well as to β–glucan, and protagen-treated
shrimp. As a conclusion, supplementation of nucleotide into shrimp pellet
enhanced nonspecific immune response and growth performance better than
β-glucan, and protagen.
Keywords: Litopenaeus
vannamei; nucleotide; total haemocyte count; PO activity; resistance; growth
Author: Henky Manoppo, Sukenda,
Daniel Djokosetiyanto, Mochamad Fatuchri Sukadi, Enang Harris
Journal Code: jpperikanangg100013
