ULTRASTRUCTURAL ANALYSIS OF GRUNT FIN (GF) CELLS TREATED WITH RED SEA BREAM IRIDOVIRUS (RSIV; family Iridoviridae, genus Megalocytivirus) IN COMBINATIONS WITH INTERFERONS AND SPLENIC SUBSTANCES
Abstract: The genus
Megalocytivirus in the family of Iridoviridae encompasses isolate of red sea
bream iridovirus (RSIV). In the present study, grunt fin (GF) cells were
treated with red sea bream iridovirus (RSIV) in combinations with interferons
(IFNs) and splenic substances. The viral titer in the combination with primary
splenic substance was higher than the other combinations of 10-1 and 10-2
diluted splenic substances, and the positive control. The viral titer was not
decreased by all combinations with recombinant murine interferon-α (rMuIFN-α),
recombinant murine IFN-β (rMuIFN-β), and recombinant feline interferon-ω
(rFeIFN-ω). Electron microscopy revealed inclusion body bearing cells (IBCs)
and enlarged cells allowing virus propagation within the intracytoplasmic virus
assembly site (VAS). Most were enlarged cells. These enlarged cells were
divided into three cell types. Cells of Type II, which contained many mature
virions within the VAS, were numerous in number in all treated cells. Cells of
Type I allowing assembly of few virions and cells of Type III containing many
immature viral particles were rather fewer in number. Their percentage was
almost the same in all combinations with the splenic substances and IFNs. These
results determined in in vitro treatment with IFNs did not prevent viral
replication of RSIV, as well as the splenic substances which were derived from
the RSIV-infected spleen of red sea bream did not contain any factors to
disturb RSIV replication.
Keywords: red sea bream;
iridovirus; grunt fin; splenic substances; interferons; ultra structural
analysis
Author: Ketut Mahardika, Teruo
Miyazaki
Journal Code: jpperikanangg100011
