PRODUCTION AND BIOACTIVITY POTENTIAL OF THREE RECOMBINANT GROWTH HORMONES OF FARMED FISH
Abstract: This study was aimed
to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus
lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus
carpio) and compare their bioactivity potential by means of inducing the growth
hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA
fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy
(Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR
products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH
protein expression vector, respectively. Each of the expression vectors was
transformed into the Escherichia coli BL21. E. coli BL21 was cultured using
2xYT medium and protein production was induced by cold shock at 15±1oC for
overnight. The inclusion bodies of E. coli transformants containing protein
expression vector were isolated by sonication method, and rGH production was
analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of
12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg
inclusion body containing rGH per gram fish body weight. The result showed that
rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of
El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively,
compared with the control. This result indicated that the three rGH produced in
E. coli possessed biological activity when tested on Nile tilapia and further
research is needed to find its effect on the growth of other aquaculture fish
species.
Keywords: bioactivity;
recombinant protein; growth hormone; Nile tilapia
Author: Alimuddin, Indra Lesmana, Agus Oman Sudrajat,
Odang Carman, Irvan Faizal
Journal Code: jpperikanangg100010
