ABSTRACT: Histology expression in asthma show inflammatory processes and airway structural changes. Treatment development in asthma needs appropriate animal models but most animal models use short term allergen exposure. The aim of this research was to create develop allergic mouse model with allergic inflammation and structural changes histology expression. Eight female BALB/c mice, 6-12 weeks old, 80-100 grams were divided into two groups. The first group were exposed to a normal saline solution where the second group received ovalbumin. Mice were sensitized by intraperitoneal injection of ovalbumin on day 0 and 14 and challenged by nebulization of 1% ovalbumin three times weekly for 6 weeks. Twenty four hours after final exposure, the mice were sacrificed by lethal cardiac puncture. The level of total IgE in serum were measured by ELISA and IL-4 receptor expression were examinated by immunohistochemistry. Morphometric analysis was performed to count inflammatory cells, eosinophil, goblet cells, epithel and smooth muscle thickness. Differences between the control and exposed mice were analyzed by Mann-Whitney test. There were a significant differences in total IgE serum and expression IL-4 receptor between the two groups (P < 0,05). The morphometric analyzing also demonstrated significant differences between these two groups (P < 0,05). Chronic ovalbumin exposure through inhalation produced allergic inflammation and airway structural changes.
Keywords: allergic inflammation, mouse models, chronic exposure, ovalbumin