JURNAL TERBARU

Aksi Ethyl Methane Sulphonate terhadap Munculnya Bibit dan Pertumbuhan Cabai Rawit(Capsicum frutescens L.)

Abstrak: Penelitian ini bertujuan  mengamati pengaruh lama perendaman biji dengan 1%  EMS  terhadap  persentase  munculnya  bibit  dan  karakter  pertumbuhan tanaman  cabai  rawit  pada  umur  4  minggu  setelah  tanam  (MST).  Benih  cabai rawit  direndam  dalam  air  selama  6  jam,  selanjutnya  direndam  1%  EMS  dalam buffer  fosfat  pH  7,  selama  6  jam,  9  jam  dan  12  jam.  Tiap  biji  disemai  dalam bumbungan  kertas.  Munculnya  bibit  diamati  setiap  hari.  Setelah  berumur  3 minggu, bibit  dipindahkan ke bedengan. Hasil menunjukkan  perlakuan  1% EMS memperlambat  munculnya  bibit.  Pada  10  hari  setelah  semai  (HSS)  munculnya bibit  pada  perlakuan  dapat  mencapai  100%.  Perlakuan  1%  EMS  berpengaruh nyata terhadap tinggi tanaman dan jumlah daun, sedangkan panjang, dan lebar daun dengan perendaman 6 jam tidak berbeda dengan kontrol, namun berbeda dengan perendaman 9 jam dan 12 jam.
Kata kunci : EMS, bibit, pertumbuhan, Capsicum frustescens L

KEANEKARAGAMAN JENIS BURUNG DI KAWASAN MANGROVE GILI SULAT LOMBOK TIMUR

Abstract: Mangrove at Gili Sulat is the important ecosystems that support the lives of  many  wildlife species, one  of  which is species of  birds. Birds use the mangroves  as searching of  food, playing, searching  for  mates,  and  nesting. The aim of  the research is to determine the diversity of  birds species  in  the mangrove area at  Gili Sulat East Lombok. The study was conducted  by observations species of  birds through surveys (direct observation) using binoculars and use the boat (during water conditions  high tide). The result of  this research have found nine species of  birds, consisting  of  four  species  of  water birds (Actitis hypoleucos, Butorides striatus, Chlidonias hibridus, and Egretta Garzetta) and five species  of  birds  terrestrial  (Lalage sueurii, Linkmera lombokia, Nectarinia jugularis, Merops ornatus, and  Zosterops  palpebrosus) .
Keywords:  Birds,  Mangroves,  Gili Sulat, East Lombok

ANALISIS TIPOLOGI LEPIDOPTERA BERDASARKAN OBSERVASI HABITAT DI KAWASAN HUTAN LINDUNG NUSAKAMBANGAN, CILACAP, JAWA TENGAH

Abstract: The objectives of this research are (1) to know abiotic component in Nusakambangan Island, (2) to know preferency of  habitat inclination Lepidoptera in Nusakambangan Island (3) to know the diversity of Lepidoptera in Nusakambangan Island.This is descriptive qualitative research. Method that used in this reseacrh consist of several stages; (1) determine observation location,(2) gathering data of abiotic and veegetation habitat of Lepidoptera, (3) gathering data of Lepidoptera composition. The result show that faound 52 species from 3 family, there are Papilionidae, Pieridae, Nymphalidae, 1 superfamiliy Hesperidae dan 1 subfamily Polyommatinae. There are 22 unidentified butterflies until species level. Habitat preference are influenced by many factors. The factors are abiotic and biotic factor. Abiotic factors include humidity, light intensity, and air temperature. For biotic factors include the of flower types, host plants.
Keywords: tipology, preferencyi habitat, lepidoptera, Nusakambangan island

STUDI DINAMIKA POPULASI KELELAWAR KUBAR JANGGUT-HITAM (Taphozous melanopogon Temminck, 1841) DI GUA SRUNGGO DI KAWASAN KARST TUBAN

Abstract: This research was aimed to determine fluctuation of  Taphozous melanopogon population in Srunggo cave in Tuban karstic area. The research began in 2010 until 2012 in Srunggo cave in Tuban karstic area of eastern Java.  This research is ecological research with this type of research, Natural Trajectory Experimment  (NTE), population-local spatial scale, and one-generation temporal scale. Data was taked by line potret method (evening emergence count modification), twice in dry monsoon and rainy season everi years. Research data analysed by descriptive. Srunggo cave is a cave with single population of  Taphozous melanopogon. Population fluctuation this species between 2010 until 2012 that are 2126, 1946, and 1832 individu. The Population trend is decrease. That caused by human disturbance in habitat and illegal hunting by local people. The population during the rainy season in three years is likely to decrease, compared to the dry season. It is possible that the species population is metapopulasi.
Keywords: Population, Bat, Taphozous melanopogon, Karst, Biospeleology

AKTIFITAS ANTIBAKTERI ACTINOMYCETES YANG DIISOLASI DARI TPA PUTRI CEMPO MOJOSONGO SURAKARTA

Abstract: Actinomycetes are procaryotic organisms which are found in many habitats, such as: land, water, and plant tissue. They have numerous function for the production of antibiotics and diversity benders to pest and disease in plants. There were many researchers explored new isolates that produces a compound of a novel metabolites. One of them is screening actinomycetes isolated  from the soil samples in Putri Cempo Landfill in Surakarta. The isolates obtained by testing the bacterial activity with well diffusion methods on the Gliserol-Yeast Extract media against four pathogenic bacteria, namely: Ralstonia solanacearum, Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. In this research obtained 10 isolates of actinomycetes, three of them have the ability to inhibit bacterial growth of R. solanacearum, one isolate having the power to against Bacillus subtilis, one isolates having the power of an abstruent against Staphylococcus aureus, and two isolates capale in inhibiting the growth of E. coli. The antibacterial activity were characterized from the inhibitory clear zone arround the ccolony of Actinomycetes. These isolates were: PC 12, PC 13 dan PC 15. The inhibitory clear zone were measured showed the power spectrum of the third of the isolates are belong to the broad spectrum due to their diameter of the clear zone is more than 8 mm.
Keywords: Actinomycetes, antibacterial activity, Putri Cempo Landfill, Streptomyces sp, clear zone

PERBANDINGAN SIFAT FISIKAWI SELULOSA BAKTERI YANG DIPRODUKSI ISOLAT KRE-12 DENGAN METODE FERMENTASI STATIS DAN PENGGOJOGAN

Abstract: Cellulose production in bacteria is influenced by the fermentation method, both quantitatively and qualitatively. Bacterial cellulose produced by different fermentation methods were compared based on the dry weight of the cellulose produced. While the physical properties compared using SEM and XRD technique. Production of cellulose in acetic acid bacteria strain KRE-12 with static fermentation method, shaking 50 rpm, shaking 100 rpm and shaking150 rpm was0,49; 0,12; 0,13 and 0,10 g/100 ml, respectively. The degree of crystallinity by XRD method in static fermentation method was 91 %, shaking fermentation  100 rpm was73 % and shaking fermentation150 rpm was72%. Static fermentation method produces bacterial cellulose in thesheets form, while shaking fermentation produces fragmented cellulose with predominantly spherical shape. The observation of the surface structure of bacterial cellulose by SEM showed that the static fermentation method generates woven densely of cellulose microfibrils. The change from a static method to shaking fermentation causes the surface structure changes. Some changes have been observed in which woven microfibrils become more loose and form a larger gap between the holecellulose woven microfibrils.
Keywords: acetic acid bacteria , bacterial cellulose, static fermentation, shaking

POTENSI BAKTERI ASAM LAKTAT SEBAGAI BIOKOAGULAN DAN KITOSAN SEBAGAI PENGAWET ALAMI DALAM PROSES PEMBUATAN TAHU BEKATUL

Abstract: Tofu rice bran is one form of diversification tofu when not socialized . Tofu as a source of vegetable protein so the impact  of  tofu as a food product that can not be stored longer that required the use of a natural preservative solution that is safe and without any residue . Deacetylation of chitin chitosan as a result polycationic nature so as to reduce the rate of growth of pathogenic bacteria . Potential of Lactic Acid Bacteria ( LAB ) as a producer of lactic acid is expected to act as a natural biocoagulant . The purpose of the study was to determine the potential of chitosan as a natural preservative and potential of LAB as natural biocoagulant  in the manufacturing process tofu  rice bran. The research method are three stages: the first stage includes : BAL isolation and cultivation of industrial products fementasi yoghurt ; the second stage includes the study of the amount of inoculum BAL ( 5 % , 10 % , 20 % ) as biocoagulant ; study the concentration of chitosan ( 0.5 % , 1 % , 2 % ) as a natural preservative and the mixtures of rice bran and soy whey ( ratio 1 : 1 , 2 : 1 , 3 : 2 ) in the process of making tofu rice bran ; The third stage involves analysis of  product tofu rice bran for fat content , protein content , fiber content and microbial contamination include MPN Coliform , Salmonella and organoleptic Hedonic test the level of preference ( color , smell , consistency , flavor ) by panelists . The results showed that tofu rice bran with 10 %  inoculum biocoagulant , concentration of chitosan 2 % as a preservative and ratio of rice bran and whey ( 2:1 ) showed  the most excellent organoleptic .
Keywords: Lactic Acid Bacteria ( LAB ) , biocoagulan t, chitosan , natural preservative , tofu rice bran

UJI AKTIVITAS ANTIBAKTERI EKSTRAK BUNGA Jasminum sambac Ait. TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923 dan Shigella flexneri ATCC 1202

Abstrak: Tanaman melati (Jasminum sambac Ait.) berpotensi dimanfaatkan sebagai  tanaman obat karena adanya senyawa metabolit sekunder pada akar, batang daun dan bunga. Penelitian ini bertujuan untuk melakukan ekstraksi dan penapisan awal senyawa aktif bunga melati yang dilanjutkan dengan pengujian aktivitas antibakterinya. Ekstraksi dilakukan dengan metode maserasi bertingkat dengan menggunakan tiga pelarut yaitu kloroform, etil asetat dan etanol. Pengujian aktivitas antibakteri dengan metode difusi agar menggunakan kertas cakram dengan pelarut etil asetat pada variasi konsentrasi 20%, 30%, 40% dan 50%. Hasil penelitian menunjukkan bahwa ekstrak bunga J. sambac Ait. mempunyai tingkat kepolaran yang berbeda. Hasil penapisan awal terhadap aktivitas antibakteri menunjukkan bahwa ekstrak etil asetat mempunyai daya hambat terbesar terhadap pertumbuhan bakteri Staphylococcus aureus dan Shigella flexneri. Zona hambat terbesar pada pengujian aktivitas antibakteri dihasilkan oleh ekstrak etil asetat dengan konsentrasi 50% terhadap bakteri S.aureus.
Kata kunci: antibakteri, ekstraksi, Jasminum sambac

ISOLASI DAN SELEKSI BAKTERI PENGHASIL BIOSURFAKTAN DARI SAMPEL LUMPUR MINYAK, KALIMANTAN TIMUR

Abstract: A total of six bacteria isolated from waste of oil sludge in the area of East Kalimantan were examined to confirm the ability in biosurfactant activity. The active compounds from each bacteria were subjected to screening test of biosurfactant by usingcanola oil as substratein fermentation system. The present study aims to evaluatedthe initial potency ofbiosurfactant producing bacteria from sludge oil in East Kalimantan. All of the isolates were confirmed as producers of biosurfactants with diverse of emulsification activity. Strain SMF4 showed the highest emulsification activity and oil displacement test within 9 days of cultivation.The screening results revealed that the six strains of sludge oil bacteria gave good values in emulsification and oil displacement activity.
Keywords: oil sludge, six bacteria, emulsification, and biosurfactant

PENGKAYAAN MIKROBA KONSORSIA PADA UREA BERLAPIS ARANG AKTIF DAPAT MEMPERCEPAT PENURUNAN INSEKTISIDA ALDRIN DI LAHAN SAWAH

Abstract: The challenge for the Indonesian nation in the future is globalization which demands a very tight competition. Three factors that support this are increased efficiency, productivity and quality of agricultural products. The use of pesticides has contributed most to the increase in agricultural production since 1970. The amount of pesticides circulating in Indonesia from year to year increases. In 2006 there are as many as 1336 registered formulations, in 2008 the amount of pesticide formulations are 1702, in 2010 there are as many as 2048 formulations, and in 2011 as many as 2247 formulations. Microbial enrichment is expected to accelerate the degradation of residual insecticides aldrin . The research is conducted at the Experiment Jakenan Station February 2012 to September 2012. The soil which is used as the planting medium is brought from the village of the District Sukamenak Rawagempol Wetan, Karawang. The experiment was conducted in field scale microplot with lysimeter, and using a randomized block design ( RBD ) 3 replications 6 fertilizer treatments ( control , prill urea , urea activated carbon maize cobs ( UAATJ ) , urea activated carbon coconut shell ( UAATK ) , urea activated carbon cob corn + microbes ( UAATJM ) , urea activated carbon coconut shell + microbes ( UAATKM ). The plants used are rice varieties inpari 13. The insecticide residue analysis is performed in the Residu Bahan Agrikimia (RBA) laboratory Balingtan. The purpose of this study is to obtain urea technology with activated carbon enriched with insecticide degrading microbes that can degrade POPs aldrin insecticide residues. microplot scale research results by using a lysimeter showed the highest reduction of residual insecticides aldrin in the treatment of urea -coated coconut shell activated carbon enriched microbial consortia that degrade POPs is 36,17 % . It is suspected that coconut shell activated carbon microbial favored as its home and the microbes utilize carbon sources of aldrin as food for growth.
Keywords: microbial enrichment , decreased aldrin , paddy field
 
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