Uji Mutagenik Ames untuk Melengkapi Data Keamanan Ekstrak Gambir (Uncaria gambir Roxb.)
Abstract: The main compound of
Uncaria gambir Roxb. (gambir), catechin and it’s derivates have been believed
to be potential as antiviral. Epigallocatechin gallate (EGCG) and epicatechin
are catechin derivates which are found to be potential as antiviral against
Human Immunodeficiency Virus (HIV). However, gambir extract also contains
quercetin that has possibility to be mutagenic. Therefore, a preliminary study
towards safety of those compounds within gambir extract, mutagenicity assay
using Ames Method has been performed.Sample (gambir extract) was obtained from
West Sumatera, Indonesia. The extract was characterized according to Farmakope
Herbal Indonesia and WHO methods. Mutagenicity test by Ames method utilized a
colorimetric microplate in 6 various concentration (125 mg/mL; 62.5 mg/mL;
31.25 mg/mL; 15.625 mg/mL; 7.81 mg/mL dan 3.91 mg/mL) against mutant bacteria
Salmonella typhimurium TA 98, Salmonella typhimurium TA 100 and Escherichia
coli WP2 uvrA with and without the addition of S-9 enzyme. Extract of gambir in
this study contains 86.60% of catechin, 12.92% moisture content, 22.49%
water-soluble extract content, 80.63% ethanol-soluble extract content, 0.81%
total ash, 0.32% acid insoluble ash content and 10.38% in dryness level. From
the mutagenicity test and calculation, fold increase (over baseline) of the
sample in 6 various concentration with and without adding S-9 enzyme are lower
than 2. Gambir extract from West Sumatra with catechin contains 86.6% hasn’t
showed mutagenic effect due to the fold increase (over baseline) of
mutagenicity test lower than 2.
Keywords: Uncaria gambir Roxb;
Ames method; Mutagenic; Catechin; HIV
Penulis: Novi Sulistyaningrum,
Lina Rustanti, Sukmayati Alegantina
Kode Jurnal: jpfarmasidd130418