STABILITAS IMUNOGLOBULIN M (IGM) CAMPAK PADA DRIED SERUM SPOTS
ABSTRACT: Shipping and storage
of measles serum specimens from fields to the reference laboratory must
consider the cold chain in order to maintain the stability of immunoglobulin M
(IgM) in serum. Meanwhile, to maintain the cold chain needs special handling.
This study aimed to determine the use of filter paper as an alternative in
shipping and storage of specimens. This experimental study used measles
positive serum received at the National Measles Laboratory Center for
Biomedical and Basic Technology of Health from January until March 2011. There
were three groups of sample. Each group consisted of 9 specimens. The effects
of temperature and storage period on the stability of measles IgM of dried
serum spots (DSS) stored in 4°C and 25°C for one, three, six, nine, twelve, and
five teen days were tested at the National Measles Laboratory Center for
Biomedical and Basic Technology of Health from July until December 2011. The
stability of measles IgM of DSS that were stored at 4°C and 25°C temperature
showed no significant difference (p = 0.316) compared to the serums stored at a
temperature of 4°C in the measurements on the third day. Dried serum spots can
not be used as sample alternative for detection of measles virus-spesific
immunoglobulin M
KEYWORDS: Dried serum spots;
Immunoglobulin M; Measles
Penulis: Kartika Dewi Puspa, Mursinah,
Ratumas Rosmawati Budianto
Kode Jurnal: jpfarmasidd130419
