UJI AKTIVITAS ANTIMIKROBA DAN IDENTIFIKASI EKSTRAK BUAH SAWO MANILA (ACHRAS ZAPOTA L.) TERHADAP BEBERAPA MIKROBA PATOGEN DENGAN METODE DIFUSI AGAR
Abstrak: The research has
conducted antimicrobial activity assay and identification of sapodilla fruit
extract manila (Achras zapota L.) against some pathogenic microbes with agar
diffusion method. Manila sapodilla fruit extraction by maceration method by
using the solvent ethyl acetate and ethanol 70%. Manila sapodilla fruit has
tested by screening extract at a concentration of 1% on the test bacteria which
is namely Staphylococcus aureus, Staphylococcus epidermis, Streptococcus
mutans, Pseudomonas aeruginosa, Salmonella thyposa, Vibrio sp, Bacillus
subtillis, and the fungus is Candida albicans. Furthermore, the inhibition has
tested by using the agar diffusion method (paper disc) at a concentratied of
1500ppm, 1000ppm, 500ppm, and 100ppm
against screening test results of bacteria. Furthermore, identification
of classes of compounds sapodilla fruit of extract phytochemicals by used
screening manila methods qualitative.
Screening results showed that the antimicrobial activity of the ethanol
extract manila sapodilla fruit can inhibit the growth of Staphylococcus aureus,
Staphylococcus epidermis, Streptococcus mutans, and Salmonella thyposa.
Inhibition test using the agar diffusion method, shows that more the
concentration used, more the diameter of the resulting barriers. The results of
the identification of sapodilla fruit ethanol extract showed a class of
compounds manila tannins, flavonoids, and terpenoids.
KATA KUNCI: antimicrobial; sapodilla fruit extract of manila; pathogenic
bacteria; agar diffusion method; Identification Compound
Penulis: Mukhriani, Nurlina,
Fajrul Fhalaq Baso
Kode Jurnal: jpfarmasidd140561
