Pengaruh Hasil Fraksinasi Ekstrak Etanol Daun Lado-lado (Litsea cubeba,Pers) Terhadap Kadar Asam Urat Serum Darah Mencit Putih Jantan Tinggi Asam Urat

ABSTRACT: Uric acid is the end product of purine metabolism in humans and imbalance of uric acid levels in the blood to triggered hyperuricemia then incidence and prevalence is quite high in Indonesia. This study was conducted to determine whether hyperuricemic affects the decrease of uric acid level in white male mouse hyperurisemia by using lado-lado (Litsea cubeba,Pers) leaf. Sample was extracted with 70% ethanol and subsequently fractionated by using hexane, ethyl acetate and water residual solvents. potassium oxonate doses 250 mg/kgBW and long bean juice (Vigna unguiculata,Walp) doses 0.5 mL/20 gramBB frequency 3 times daily as inducer. It taked for 5 days to hyperuricemia condition. At the time of treatment, only use long peanut juice for inducer. Doses 12,5 mg/kgBW; 25 mg/kgBW and 50 mg/kgBW and variations of 7, 14 and 21 days on hyperuricemic condition. Serum uric acid levels were measured by using a clinical photometer with an enzymatic principle. The resultant decrease in uric acid levels was best obtained at the fraction of ethyl acetate of lado-lado leaves (<0.05) and the doses of 50 mg/kgBW with 7 days of administration was the best variation to decrease uric acid levels in high uric acid serum level condition.
Kata kunci: daun lado-lado; asam urat; fraksinasi; fotometer; hiperurisemia.
Penulis: Rizky Yulion P, Suhatri, Helmi Arifin
Kode Jurnal: jpfarmasidd170148

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