OPTIMASI PRODUKSI ANTIBIOTIKA ISOLAT TERPILIH FUNGI ENDOFIT IKD FF-UMI 02 DARI KULIT BUAH DELIMA (Punica granatum L.) DENGAN VARIASI SUMBER KARBON
Abstrak: Research of
endophytic fungi of pomegranate fruit peel (Punica granatum L.), as Anti-free
radicals and antibiotic has been done with DIKTI-PKM funding at 2014. It is
considered necessary to further research about optimization of antibiotic
production from pomegranate fruit peel with variation of carbon source, first
step is selection of isolates that have largest inhibition zone and has the
most inhibit microbial test, that is endophytic fungi IKD FF-UMI 02 that has
activity on Salmonella thypii, Staphylococcus epidermidis, Staphylococcus
aureus and Shigella dysenteriae. Determination of growth curve from isolate by
inoculating into Potato Dextrose Broth and incubated for 7x24 hours. Every 12
hours the mycelium is weighed to determine its stationary phase. Determination
of optimum conditions of fermentation with carbon source such as glucose,
galactose, and fructose. Endophytic fungi IKD FF-UMI 02 inoculated and
fermented into production medium which is every 12 hours fermentate was taken.
The activity was tested using agar diffusion method in Nutrient Agar (NA)
against Salmonella thypii, Staphylococcus epidermidis, Staphylococcus aureus
and Shigella dysenteriae. Resulted that growth curve of selected endophytic
fungi IKD FF-UMI 02 from pomegranate fruit peel show that stationer phase
starting on 192 hour and galactose carbon source is show optimum time of
antibiotic production on 120 hours with inhibition zone 10,24 mm against
Shigella dysenteriae.
Keywords: Optimization,
antibiotic production, endophytic fungi IKD FF-UMI 02, Pomegranate fruit peel,
Punica garanatum L., carbon source
Penulis: Rusli, Nurul Vicky
Syfriani, Sumarni Hatta, Muh. Wais
Kode Jurnal: jpfarmasidd170405
