IDENTIFIKASI SENYAWA METABOLIT SEKUNDER DAN UJI AKTIVITAS EKSTRAK ETANOL HERBA MENIRAN (Phyllanthus niruri L.) TERHADAP CANDIDA ALBICANS MENGGUNAKAN METODE DIFUSI CAKRAM
Abstract: The aim of this
research is to know the ability of meniran ethanol extract (Phyllanthus niruri
L.) in inhibiting the growth of Candida albicans. The research design is an
experimental study. The extract used was herbal meniran extract made by
maceration using 70% ethanol solvent, the content of ethanol extract of meniran
tested against Candida albicans using disc diffusion method were 5%, 10%, 20%,
30% and 40%. Activity test conducted on Sabaraound Dextrose agar media that has
been smeared with Candida albicans culture which has been standardized with Mc
Farland standard. The pre-tested disc paper is first dipped into the extract
with various concentrations. Incubated at 30 ° C for 24 hours and the inhibit
zone formed was measured. The result showed that herbal ethanol extract of
meniran resulted in drag zone diameter at Candida albicans ie 8,5 mm; 10.3 mm;
12.6 mm; 14.1 mm and 14.3 mm, for negative control 0 mm and positive control
20.1 mm. Data obtained from the results of the study were analyzed using one
way anova method showed p - value <0,05, it was concluded that there was
significant difference between negative control, 5%, 10%, 20%, 30%, 40% and
positive control.
Keywords: Meniran (Phyllanthus
niruri L.), Candida albicans, Disc diffusion
Penulis: Anita Apriliana, Akademi
Farmasi Samarinda, Eko Kusumawati
Kode Jurnal: jpfarmasidd170098
