Evaluasi Penggunaan Kromatografi Lapis Tipis Kinerja Tinggi (KLTKT) Densitometri Silika Gel 60 F254 Pada Penetapan Kadar Vitamin C Yang Terdapat Pada Daging Buah Naga Ungu (Hylocereus polyrhizus)
Abstrak: Determination of
vitamin C in purple dragon fruit (Hylocereus polyhizus) using High Performance
Thin Layer LiquidChromatography (HPTLC) Densitometri with silica gel 60 F254
plate has been studied. The flash of dragon fruit was extracted using methanol
by maseration method and the maseration extract was concentrated by using
rotary evaporator. Vitamin C was seperated
by using HPTLC plate with silica gel 60 F254 as stutionary phase and
etanol : acetic acid (9.5 : 0.5) as mobile phase and the Rf was 0.64. The spot was maesured by
using TLC-densitometry at 254 nm. The vitamin C content in dragon fruit
maesured by using linear reqression y = 1726.9 + 17.8008x, r = 0.992. Limit of
detection (LOD) and limit of quantification (LOQ) ware 13.32360 ppm and
44.41201 ppm. Respectively the recovery
and RSD were calculated which, were 99.24 % and 7.42 % respectively. The study
result showed that vitamin C content was 31.21564 ± 2.58116 ppm.
Kata Kunci: Vitamine C; Purple
dragon Fruit; HPTLC; Densitometry
Penulis: Ridho Asra,
Zulharmita Z, Muhammad Amrul
Kode Jurnal: jpfarmasidd170364
