AKTIVITAS ANTIMIKROBA EKSTRAK DAUN TEH HIJAU (Camellia sinensis) DAN JATI BELANDA (Guazuma ulmifolia Lamk.)TERHADAP MIKROBA PATOGEN SECARA BIOAUTOGRAPHY-TLC
ABSTRACT: An investigation has
been conducted on the antimicrobial activities of extract teh hijau leaves
(Camellia sinensis) and jati belanda leaves (Guazuma ulmifolia Lamk.) using
TLC-Bioautography. The aom of this research is to test the antimicrobial
activity of extract against some pathogen microbes. The preliminary research
conducted screening test using several tipe of microbes including Bacillus
subtilis, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Shigella
dysentriae, Staphylococcus aureus, Staphylococcus epidermidis, Strepthococcus
mutans, and Vibrio cholera. The screening results of extract inhibited almost
all of pathogen microbies, except Bacillus subtilis and Staphylococcus aureus
for extract teh hijau leaves and Vibrio cholera, Staphylococcus aureus, and
Escherichia coli for extract jati belanda leaves. The research was continued
with the antimicrobial activity against severeral microbes at different Rf and
the result showed that the higher inhibited at Staphylococcus epidermidis Rf
0,02 and Shigella dysentriae Rf 0,49, 0,73 and 0,8 for teh hijau leaves,
Pseudomonas aeruginosa Rf 0,02 dan 0,49 for jati belanda leaves. That extracts
have antimicrobial activity to several pathogen microbes, so that are can use
as one of alternative antimicrobial.
Keywords: Teh Hijau leaves
(Camellia sinensis), Jati Belanda leaves (Guazuma ulmifolia Lamk.),
TLC-Bioautography, Antimicrobial
Kode Jurnal: jpfarmasidd170403