Immune-Enhancing Effect of Ethanol Extract of Pegagan Herb (Centella Asiatica Urban) on Rat
Abstract: This research had
been conducted to verify the activity of ethanolic extract of Pegagan or Centella
asiatica urban (CA) from indonesia as immune enhancing with the activity and
capacity ofperitoneal macrophages as parameters tested. Sprague Dawley male
rats were divided into 4 groups of 5 rats each. Dose 1, dose 2 and dose 3
groups were treated with extract at the dose of 10, 20 and 40 mg/200g b.w. p.o.
for 15 consecutive days, respectively, and the control group was supplemented
with carrier.one day after the last treatment, all rats were sacrifced after
induced by Staphylococcus epidermidis (109CFu per rat) i.p. and then the
activity and capacity of peritoneal macrophages were examined with giemsa staining
microscopically. The results showed that the immune-enhancing effect of the
extract was a dosedependent manner. based on statistical analysis (ANoVA, p
< 0.05), the administration of CA extract atthe dose of 40 mg/200 g bw
demonstrated the highest result and differed from control group signifcantly(96%
of macrophage activity and 61 bacteria of macrophage capacity). From these
experiments couldbe concluded that the ethanolic extract of CA had potential to
be developed as immune-enhancing agent.however, it was still needed to conduct
further study for elaborating the mechanism of immune enhancer completely.
Keywords: activity and
capacity macrophage, Centella asiatica urban, Staphylococcus epidermidis, rat
Penulis: Sri NiNgSih, AguNg
Eru WiboWo
Kode Jurnal: jpfarmasidd110190
