Vitamin D Prevents Endothelial Damage Induced by Increased Neutrophil Extracellular Traps Formation in Patients with Systemic Lupus Erythematosus
Abstract: to investigate the
effects of Vitamin D calcitriol/1,25(OH)2D3 on NETosis in systemic lupus
erythematosus (SLE) patients with hypovitamin D. Methods: neutrophlis of five
SLE patients with hypovitamin D were divided into 4 groups, P0 (0 nM/control),
P1 (1 nM), P2 (10 nM), and P3 (100 nM) as cultured samples. Phorbol Myristate
Acetate (PMA) was used to stimulate NETs formation. The supernatant was
separated and cocultured with HUVECs. Externalization of Neutrophil Elastase
(NE) and Myeloperoxidase (MPO) during NETosis was measured by
immunofluorescence and ELISA respectively. Early and late apoptosis of
endothelial cell was measured by flowcytometry using cell death kit (Annexin V
and PI antibody). Results: this study showed significant decrease in early
apoptosis with 10 nM of 1,25(OH)2D3 compared to control group. Significance of
NE externalization found in all treatment groups (p<0.05), while MPO
absorbance in the same tendency but not statistically significant. Further
analysis also found a moderate positive correlation between NE externalizations
with early apoptosis. Conclusion: vitamin D 1,25(OH)2D3 could reduce
endothelial damage by decreasing NETosis activity. This result may reveal the
possibility of Vitamin D as supplementary therapy for SLE patients with
hypovitamin D to prevent endothelial damage.
Key words: NETs, NE, MPO,
1,25(OH)2D3, HUVECs
Author: Kusworini Handono,
Yona One Sidarta, Benny A Pradana, Radhitio A Nugroho, Ivan A Hartono, Handono
Kalim, Agustina T Endharti
Journal Code: jpkedokterangg140321
