The Role of Anti-dengue Virus NS-1 and Anti-protein Disulfide Isomerase Antibodies on Platelet Aggregation in Secondary Dengue Infection
Abstract: to observe the
correlation between anti-NS-1 and anti PDI antibodies against platelets
function disorder on secondary dengue infection. Methods: 50 patients with
secondary DV infection according to WHO criteria were observed by a cross
sectional study. Patient’s blood was collected on day 3, 5 and 7 after fever
onset. Platelets aggregation test was done to prove the possibility of
platelets dysfunction. Anti-NS-1 and anti- PDI antibodies were determined by
solid phase ELISA. Results: the inhibition of platelets aggregation was
increased among day of observation. Means value of inhibition on day 3 is
46.6%, day 5 is 52.5% and day 7 is 56%. There is a significant difference
(p<0.05) of inhibition of platelet aggregation value between days of observation.
The antibodies against NS-1 DV and PDI were detected in all 50 sera with the
positive rate of 90% develop NS-1 antibodies and 72% of PDI antibodies, on day
3 of symptoms. The highest OD of NS-1 antibodies is detected on the day 3 and
decreased on day 7. The OD of PDI antibodies was increased on day 3 and still
increasing on day 7. There is a significant correlation between anti NS-1 and
PDI antibodies (r=0.386-0.490), while the differences of OD between observation
days are not significant (p>0.05). Conclusion: the kinetics profile of NS1
and PDI antibodies responses, which were detected by the third day of symptoms.
Dengue patients’ sera inhibited platelets aggregation. NS-1 antibodies and PDI
antibodies might have a role on the platelets aggregation dysfunction; however,
there is no correlation between them. It is possible that other mechanism
involve in the inhibition of platelets aggregation.
Key words: anti-NS-1
antibodies, anti PDI antibodies, inhibition platelets aggregation, secondary
dengue infection
Author: Andhika Rachman, A.R.
Harahap, R.M. Widhyasih
Journal Code: jpkedokterangg130281
