Phenotype Characterization of Beta-Lactamase Producing Enterobacteriaceae in the Intensive Care Unit (ICU) of Cipto Mangunkusumo Hospital in 2011
Abstract: the goal of this
study is to understand the phenotype characteristic of beta-lactamase enzymes
producing Enterobacteriaceae, such as ESBL, AmpC, and carbapenemase. Methods:
three different methods are performed to confirm those phenotypic
characteristics, namely double disk diffusion method to confirm ESBL, AmpC disk
test (cefoxitin-based) to confirm AmpC, and modified Hodge test to confirm
carbapenemase. Results: using double disk diffusion method, we found 58.42%
isolates are ESBL-producing, whereas the outcomes of AmpC disk test shows 1.98%
are AmpC-producing. By conducting modified Hodge test (MHT), 27.59% isolates
are confirmed as carbapenemase-producing bacteria. Conclusion: this study
confirmed the prevalence of beta-lactamase producing Klebsiella pneumoniae is
very high. Nevertheless, AmpC and carbapenemase should not be ignored despite
their low prevalence.
Key words: Enterobacteriaceae,
antibiotic resistance, ESBL, AmpC, carbapenemase
Penulis: Yulia R Saharman,
Delly C Lestari
Kode Jurnal: jpkedokterangg130276
