Induction of Rat Germ Cell Apoptosis by Testosterone Undecanoate and Depot Medroxyprogesterone Acetate and Correlation of Apoptotic Cells with Sperm Concentration
Abstract: to investigate the
safety and effectiveness of dose TU+DMPA hormones in developing potential male
contraceptives. Methods: suppression of rat sperm concentration through
increased apoptotic germ cells by in vivo administration of a long-acting androgen
composed of a combination of testosterone undecanoate (TU) and depot
medroxyprogesterone acetate (DMPA) was performed. Adult Sprague-Dawley rats
received 2.5 mg TU every 6 week and 1.25 mg DMPA every 12 week for 60 week, a
regimen known to rapidly reduce testosterone production by the testes and
produce azoospermia within 12 week. Sperm concentration data were log
transformed before analysis. Results are expressed as the mean±SEM. ANOVA,
followed by post hoc test was used to determine differences across time and
phase. T test was employed to determine differences between two groups.
Results: apoptosis revealed significant increase in apoptotic germ cells (80%
when rats were administered with TU+DMPA. Apoptotic germ cells can be found in
several spermatogonia (20%), spermatocytes (30%), and spermatids (50%).
Conclusion: dose TU+DMPA hormones may be a safe and effective way to develop
potential male contraceptives.
Key words: TU, DMPA, sperm
concentration, apoptosis
Author: Syafruddin Ilyas,
Silvia W Lestari, Nukman Moeloek, Asmarinah Asmarinah, Siregar C Siregar
Journal Code: jpkedokterangg130279
