THE EFFECTS OF ETHANOLIC EXTRACT OF GREEN ALGAE (ULVA LACTUCA L.) ON BLOOD CHOLESTEROL LEVELS IN MALE RATS INDUCED BY A HIGH FAT DIET
Abstract: Nowadays,
cardiovascular disease is increasing in number each year. It is triggered by
high cholesterol diet consumed by urban people. Green algae (Ulva lactuca L.)
contains phytomelatonin levels that can ease cholesterol.
Objective : To know about the effect of giving green algae ethanolic extract
in male strain Wistar mice’s cholesterol level.
Methods : This research involved 36 male strain Wistar mice divided to
six groups. Negative control injected with 2 ml/200 gBW lard, positive control
injected with 0,18 mg/200 gBW simvastatin and lard, group III, V, V each given
lard and injected with ethanolic extract of green algae at a dose of 50
mg/kgBW, 100 mg/kgBW and 200 mg/kgBW respectively, and normal control given AD
2 dietary treatment. Each group was given the same treatment peroral in 56 days
and the blood was taken every 14 days through sinus orbitalis. The blood
cholesterol level was analyzed by using enzymatic CHOD-PAP method. Data
obtained were statistically examined using Two way ANOVA , Kolmogorov Smirnov,
Levene’s method and LSD with confidence level at 95%.
Results : The blood cholesterol level were not significanly different
between the negative and normal control. The result of cholesterol level for
14, 28, and 42 days for all group did not increase compared to negative
control, but cholesterol level in group with 200 mg/kgBW dose extract indicated
significant difference with negative control for 56 day.
Conclusion : Giving ethanolic extract of green algae for 56 days
decreased blood cholesterol level in male rats.
Keywords: green algae,Ulva
lactuca, Lcholesterol,lard
Author: Wahyu Widyaningsih
Journal Code: jpkedokterangg160334
