THE EFFECT OF GREEN TEA LEAF EXTRACT ON SPATIAL MEMORY FUNCTION AND SUPEROXYDE DISMUTASE ENZYME ACTIVITY IN MICE WITH D-GALACTOSE INDUCED DIMENTIA
Abstract: Oxidative stress and
inflammation play an important role in pathogenesis of brain aging and
neurodegenerative diseases such as Alzheimer. Green tea has been shown to have
antioxidant, anti-inflammatory, anticancer, and neuroprotective activity.
Objectives: to determine the effect of green tea extract on spatial memory
function and superoxide dismutase enzyme activity in mice with D-galactose
induced dementia
Methods: An experimental study using "post test only control group
design". Twenty male BALB/c Mice aged 6-8 weeks were divided into 4
groups. Negative control group (NG) was induced by subcutaneous injection of
D-galactose (150 mg/kg BW) once daily for 6 weeks. GT-90, GT-270, GT-540 were
induced by D-galactose and orally administered with 90, 270, and 540 mg/kg BW
of green tea extract once daily for 6 weeks. The spatial memory functions were
assessed using Morris water maze and SOD enzyme activities were evaluated using
ELISA. One-way Anova and Kruskal-Wallis were used for statistical analysis.
Results: mean percentage of latency time in the GT-90 (35.29 (SD=
2.69)%), GT-270 (35.28 (SD= 2.62)%), and GT-540 (35.62 (SD=5.05)%) were
significantly higher compared to that of NG (20.38 (SD = 3.21)%), p <0.05).
SOD enzyme activity in the GT-270 (0.78 (SD = 0.07) U/ml) was significantly
higher compared to that of NG (0.51 (SD = 0.01) U ml), p= 0.004).
Conclusion: Green tea extract may improve spatial memory function and the
activity of superoxide dismutase enzyme in mice with D-galactose induced dementia.
Keywords: Green tea extract;
spatial memory function; SOD; D-galactose
Author: Ainun Rahmasari Gumay,
Saekhol Bakri, Astika Widy Utomo
Journal Code: jpkedokterangg160205
