In Vitro Effect of Alfa Mangostin on Multiresistant Uropathogenic Escherichia Coli
ABSTRACT: In Indonesia, the
most commom uropatogen E. coli resistance has been to ampicillin (91.9%),
ciprofloxacin (83.7%) and cefixime (67.6%). α-mangostin, a chemical compound,
has been developed as a new antibiotics isolaated from herbal Garcinia
mangostana L, but its effectiveness against multiresistant uropathogenic E.
Coli has not been established. Objective: This study examined the effect of
α-mangostin on growth of multiresistant E. coli
Methods: α-mangostin Treatment of E. coli uropatogen bacteria was
administered in vitro, using 14 levels of concentration 14; 28,13; 56.25;
112.5; 225; And 450 μg/mL with 4 times replication at each concentration. The
antibacterial activity of α-mangostin was determined by evaluating bacterial growth
at each concentration using the indirect method by sample absorbance reading.
The Samples of uropatogen of E. coli treated with various doses of α-mangostin
were incubated for 18-20 hours and then subjected to the absorbance reading
using a UV-Vis spectrophotometer λ 625 nm.
Results: Minimum inhibitory concentration (MIC) in this study was 450
mg/mL. Based on linear regression (STATA 13.1) relationship between α-mangostin
concentrations and bacterial growth inhibition activity showed 0.0001 <0.05
showing that all concentrations of α-mangostin simultaneously had a significant
effect on the growth of uropathogenic E. coli.
Conclusion: α-mangostin has not been effective to inhibit the growth of
multiresistent uropathogentic E. coli due to a relatively high MIC (450
mcg/mL). a Potentially relevant activity in the clinical setting will occur if
the value of the MIC of a substance in vitro <100 μg /mL. Even the
pharmaceutical industry prefers the development of antibiotics with in vitro
MIC value of ≤ 2 μg/mL.
Keywords: MDR, antibiotics,
Garcinia mangostana L
Author: Maya Dian Rakhmawatie ,
Afiana Rohmani , Fariz Wafaul Ahyar
Journal Code: jpkedokterangg160206
