Propolis increases neuronal count in hippocampal area CA1 and prefrontal cortex in stressed rats
Abstract: Stress induces
neuronal cell damage in the hippocampus and prefrontal cortex. Propolis has a
neuroprotective effect that can inhibit apoptosis and decrease neuronal cell
count. This study aimed to determine the effect of propolis on neuronal cell
count in hippocampal area CA1 and prefrontal cortex in Sprague Dawley rats with
induced stress.
Methods
A study of laboratory experimental design was conducted involving 24 male
Sprague-Dawley Rattus norvegicus. The animals were randomly divided into 4
groups, i.e. controls (K), and stress groups P1, P2 and P3. Controls did not
receive treatment, stress group (P1) received stress treatment, groups P2 and
P3 received stress and propolis at 100 and 200 mg/kgBW, respectively. Stress
and propolis were given for 14 days, followed by termination. The number of
neurons in the hippocampal area CA1 and prefrontal cortex were counted. One way
ANOVA was used to analyze the data.
Results
The neuronal count in the hippocampal area CA1 and prefrontal cortex in
the stress group (P1) was lower than in groups K, P2 and P3. There were
significant differences in the neuronal count of the hippocampal area CA1
between P1 and P3 and P1 and K (p=0.019) and also in the neuronal count of the
prefrontal cortex between P1 and P2, P3 and K (p=0.002).
Conclusions
This study strongly suggest that propolis inhibits the decrease in
neuronal count in in the hippocampal area CA1 and prefrontal cortex of Sprague
Dawley rats with induced stress. The present study suggests a potential
neuroprotective effect of propolis in the prevention of neurodegenerative
disorders.
Keywords: Propolis;
hippocampus; prefrontal cortex; stress; rats
Author: Kuswati Nugroho, Ety
Sari Handayani, Zainuri Sabta Nugraha
Journal Code: jpkedokterangg170055
