Permanent flame-blunted monofilament of middle cerebral artery occlusion technique for ischemia stroke induction in animal models
Abstract: Rat is the most
frequently used animal for ischemic stroke studies. Recently, middle cerebral
artery occlusion (MCAO) by introducing various types of surgical monofilament
intraluminally has been widely used, with their advantages and disadvantages.
For permanent occlusion, problems with mortality in rats are higher than
transient. In this study, we used permanent occlusion using modified
monofilament by flaming on its tip which may reduce mortality rate, so that
chronic phase of stroke can be learned extensively.
Methods: Three male Sprague-Dawley rats underwent permanent MCAO. The flame-blunted
monofilament was introduced through common carotid artery. Hematoxylin eosin
histopathology confirmation and functional assessment post-stroke induction
were then evaluated.
Results: Evaluation was conducted on 3 rats in different time post-stroke
induction (48 hours, 72 hours, and 3 weeks). Using histopathological
examination, the infarction was proved in all 3 rats showing red neurons,
perivascular edema and neutrophil spongiosis, in infarct and peri-infarct area.
The changes in histopathology showed spongiosis were more dominant in 3
week-post-MCAO rats. On the other hand, red neurons and perivascular edema were
less compared to 48 and 72-hour-post-MCAO rats.
Conclusion: Flame–blunted monofilament showed its efficacy in producing
infarct area. The advantages of this technique are easy to perform with simple
and less expensive modification of the monofilament. Conducting successful
permanent occlusion with less mortality rate will give chances to do further
research on stroke in chronic phase and its effect on novel treatment.
Keywords: middle cerebral
artery occlusion; monofilament; rat
Author: Yetty Ramli, Ahmad S.
Alwahdy, Mohammad Kurniawan, Berry Juliandi, Puspita E. Wuyung, Yayi D.B.
Susanto
Journal Code: jpkedokterangg170219
