Malondialdehyde levels are higher and glutathione levels are lower in preeclampsia than in normal pregnancies
Abstract: Maternal mortality
rate is still a health problem in Indonesia. One major contributor to maternal
mortality rate in Indonesia is preeclampsia. One widely accepted theory is that
preeclampsia is caused by oxidative stress. Placental hypoxia or ischemia among
preeclampsia patients is thought to be the cause of the formation of free
radicals such as malondialdehyde (MDA), which decreases endogenous antioxidants
such as glutathione (GSH). This study aims to ascertain the difference in
plasma malondialdehyde and glutathione levels between healthy and preeclamptic
pregnant women.
Methods
This was an observational analytic study of cross sectional design.
Research subjects were 30 normal
(healthy) pregnant women (NP), and 30 pre-eclamptic pregnant (PE) women. The
measurement of plasma MDA and GSH levels was done at the Biochemistry and
Molecular Biology laboratories, Faculty of Medicine, Sriwijaya University using
Sigma-Aldrich MDA and GSH assay kits. Mann Whitney test was used to analyze the
data.
Results
Subjects aged >35 years, with age of gestation >35 weeks and
multipara was significantly higher in the PE group compared to the NP group
(p=0.016; p=0.01 and p=0.36; respectively). MDA level was significantly higher
in the PE group than in the NP group (p=0.002). In contrast, GSH level was
significantly lower in the PE group than in the NP group (p=0.003).
Conclusion
MDA and GSH may reflect vascular complications of PE, and the ensuing
increases in lipid peroxidation may play important pathogenic roles.
Keywords: Free radical; GSH;
normal pregnant; MDA; preeclampsia
Author: Subandrate, Mia Esta
Poetri Afdal Faisal, Nurul Windi Anggraini, Sadakata Sinulingga
Journal Code: jpkedokterangg170053
