Kualitas, Ekspresi HSP 70 dan Kerusakan DNA Spermatozoa Post Thawing Sapi Limosin Pasca Pendinginan pada Suhu 5°C

Abstract: Good quality frozen semen is one of the factors that contribute to successful artifcial insemination. To get high fertility, spermatozoa should be added with extenderand cooled at 5°C for some time after collection. This study was aimed to know spermquality variables including motility, viability, abnormality, and plasma membraneintegrity as well as the expression of HSP 70 and the DNA damage of frozen semen after cooling at 5°C for 4 hours and 22 hours one limousine bull. Semen was collected from one Limosin bull twice a w week using artifcial vagina. Following colection, semenwere evaluated macroscopicly and microscopically including viability, abnormality andplasma membrane integrity. Semen then cooled at 5°C for 4 and 22 hours. Before freezing,semen was cooled at 5°C for 4 and 22 hours. Motility, viability, abnormality, plasmamembrane integrity, expression of HSP 70 and DNA damage were evaluated before and after freezing. In before freezing evaluation showed signifcant difference (p<0,05) onmotility beetween cooling at 5°C for 4 and 22 hours (55.6 ± 1.8 and 58,8 ± 2.3 % respectively), but there was no diference on viability, abnormality and plasma membraneintegrity, HSP 70 expression and DNA damage. Meanwhile, in post thawing no diference was found on motility, viability, abnormality, plasma membrane integrity, expression ofHSP 70 and DNA damage
Keywords: cooling at 5°C, HSP 70 , DNA damage
Penulis: Anny Amaliya, Suzanita Utama, Hardijanto
Kode Jurnal: jppeternakandd170049

Artikel Terkait :