Isolated Bacteria from Hemodialysis Water Distribution Systems in Hemodialysis Centers in Bandung
Abstract: Despite the advent
of water treatment technology for the past few decades, bacterial contamination
is still an everlasting issue that requires solid intervention. Many studies
across the world have identified myriad of bacteria that colonize the
hemodialysis water distribution system. This study was conducted to identify
common bacteria that colonized the hemodialysis water distribution systems in
Bandung.
Methods: This was a descriptive laboratory study conducted at the
Department of Microbiology Faculty of Medicine Universitas Padjadjaran in 2014.
Sterile bottles were used to collect 16 samples of reverse osmosis water from 2
hemodialysis centers in Bandung. Approximately 15 ml of water volume was
collected in each bottle from 7 standard points for water sampling in
hemodialysis system. The samples were first inoculated into R2A agar by
pour-plate method and colonies were sub–cultured onto MacConkey and blood agar
and the identification was based on Gram stain morphology, colony
characteristics, and biochemical tests.
Results: Micrococcus luteus and Pseudomonas sp. were the two predominant
organisms which colonized the hemodialysis water distribution system. In
addition, some genus of the Enterobacteriaceae such as Enterobacter aerogenes,
Klebsiella pneumoniae, and Yersinia pseudotuberculosis were also isolated from
the system and seemed to colonize the entire system.
Conclusions: Bacterial contamination in hemodialysis water distribution
system in Bandung is still a major problem regardless the efforts taken to
minimize it.
Keywords: Bacteria,
hemodialysis, reverse osmosis, water
Author: Mohd Hafeez bin Mohd
Rafee, Sunarjati Sudigdoadi, Julius B Dewanto
Journal Code: jpkedokterangg160239
