Identification of Pregnancy-Associated Glycoprotein (PAG) on Jawarandu Goat Cotyledons

ABSTRACT: Pregnancy-Associated Glycoprotein (PAG) is a specific glycoprotein which associated to pregnancy. It is secreted by embryo as a signal of pregnancy before implantation. The aim of the studywas to identify the molecular weight of PAG in Jawarandu goat. Placentas were obtained after laborto made the cotyledon extract. The study conducted through four stages, namely the extraction,purification performed using acid precipitation (H3PO4 1 M, pH 4.7) and salt precipitation ((NH4)2SO440% dan 80%), filtration using Sephadex G-75®, and identification the molecular weight of PAG usingSDS-PAGE. Protein bands appeared on SDS-PAGE in the K-8 column showed two protein bands with molecular weight namely 43.61 kDa and 28.21 kDa. These two molecule could be used as a marker ofearly pregnancy in Jawarandu goat.
Keywords: PAG, Cotyledons, Jawarandu Goat, SDS-PAGE
Penulis: K. A. Permana, E. T. Setiatin dan D. Samsudewa
Kode Jurnal: jppeternakandd130389

Artikel Terkait :