Identification of Pregnancy-Associated Glycoprotein (PAG) on Jawarandu Goat Cotyledons
ABSTRACT: Pregnancy-Associated
Glycoprotein (PAG) is a specific glycoprotein which associated to pregnancy. It
is secreted by embryo as a signal of pregnancy before implantation. The aim of
the studywas to identify the molecular weight of PAG in Jawarandu goat.
Placentas were obtained after laborto made the cotyledon extract. The study
conducted through four stages, namely the extraction,purification performed
using acid precipitation (H3PO4 1 M, pH 4.7) and salt precipitation ((NH4)2SO440%
dan 80%), filtration using Sephadex G-75®, and identification the molecular
weight of PAG usingSDS-PAGE. Protein bands appeared on SDS-PAGE in the K-8 column
showed two protein bands with molecular weight namely 43.61 kDa and 28.21 kDa.
These two molecule could be used as a marker ofearly pregnancy in Jawarandu
goat.
Keywords: PAG, Cotyledons,
Jawarandu Goat, SDS-PAGE
Penulis: K. A. Permana, E. T.
Setiatin dan D. Samsudewa
Kode Jurnal: jppeternakandd130389
