Identification of Pathogen Protozoans (Cryptosporidium spp and Giardia lamblia) from Canteen’s Water-sources at Jatinangor, West Java, Indonesia
Abstract: The prevalence of
intestinal protozoan infection caused by Cryptosporidium spp and Giardia
lamblia has been rapidly increasing in developing countries. Contamination of
water-sources by intestinal protozoans can spread infection to humans when it
is consumed without any specific treatment. Jatinangor is an educational area
where many universities are located and many students live in this area. This
study aimed to identify the presence of intestinal protozoa (Cryptosporidium
spp and Giardia lamblia) in water-sources used by canteens at Jatinangor area
to detect the magnitude of protozoans intestinal infections.
Methods: A descriptive cross sectional study was carried out from August
to September 2014. Raw water samples from reservoir tanks, water containers and
tapwater were taken from all canteens located along the main road of
Jatinangor. Samples were centrifuged and divided into two portions; two drops
from the first test tube was stained with Lugol solution and observed by 2
observers under a light microscope for Giardia lamblia, and sediments from the
second test tube was stained using acid fast staining then observed for
Cryptosporidium spp under the light microscope.
Results: Out of 50 samples, 26 were positive for parasites, of which 22
(44%) were contaminated by Cryptosporidium spp and 4 (8%) were contaminated by
Giardia lamblia.
Conclusions: Half of the raw water-sources in Jatinangor are contaminated
by Cryptosporidium spp and Giardia lamblia. Proper water treatment should be
implemented by the owners of the canteens before it is consumed as drinking
water.
Keywords: Cryptosporidium spp,
Giardia lamblia, water-source
Author: Komathi Nair
Murugesan, Ridad Agoes, Sri Yusnita Irda Sari
Jounal Code: jpkedokterangg170143
