Genotype analysis of partial growth hormone gene (GH891│MspI) in Pesisir cattle and Simmental-Pesisir crossbred cattle
Abstract: Growth hormone gene
that controls metabolism and body size of animals and polymorphism of the gene
is known related to performance of livestock. Identification of gene
polymorphisms was important to get early information to determine genetic
markers associated with economically desirable traits. Genetic mapping is one
of important characterizations in the selection process. Pesisir cattle is one
of local cattle in Indonesia that are reared in West Sumatera and have become
adaptive to local environment. The present study aimed to identify the genotype
of Growth Hormone gene (GH891│MspI) of the Pesisir cattle and crossbred
Simmental-Pesisir cattle (designated as SimPes) and relationship between growth
performance (body weight and body size). The present study was conducted to 30
blood samples consisted of 15 Pesisir cattle and 15 SimPes cattle. Body weight
and body size were measured at 12-18 months of age. The Polymerase Chain
Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method was used in
this research. The results showed that there exist three variant genotypes
(MspI-/-, MspI+/-, MspI+/+) and that allele frequencies of MspI- and MspI+ were
53.3% and 46.7%, respectively. Non-significant effect has found between GH’s
genotype and growth traits (Body measurement and body weight) in both of breed
cattle. In conclusion, three variant genotypes (GH891│MspI) in Pesisir and
SimPes (Simmental x Pesisir) cattle population were found and no significant
effect on growth trait performance.
Keywords: genotype; MspI
enzyme; growth hormone gene; growth trait; Pesisir cattle; SimPes cattle
Author: T. Hartatik, D. E.
Putra, S. D. Volkandari, T. Kanazawa, S. Sumadi
Journal Code: jppeternakangg180005