Evaluation of semen quality of buffalo frozen semen produced by Artificial Insemination Center
Abstract: This study aimed to
evaluate and compare the quality of post thawing spermatozoa of buffalo frozen
semen produced by artificial insemination centers on standard values, and
proposed reference values (PRV). Materials of the research were 60 samples of
straws obtained from three Artificial Insemination Center, which are each 20
straws, respectively. Parameters observed were motility, concentration,
longevity, plasma membrane integrity (PMI), acrosome integrity (AIn) and
recovery rate. The obtained data were tested by Z test then presented as mean ±
standard deviation. The research results from three artificial insemination
center showed that motility mean was 45.00±3.07%, concentration mean was 26.09±3.11
x106 cells/0.25mL, longevity mean was 10.38±0.75%/hour, PMI mean was
45.86±10.67%, AI mean was 73.99±5.28% and recovery rate mean was 64.38±5.16%.
The conclusions of this research were the motility and concentration mean were
higher than Indonesian National Standard (INS), longevity mean was lower than
Department of Animal Husbandry, Dairying and Fisheries (DADF) standard but PMI
and AIn mean were higher, the mean value of recovery rate was higher than of
proposal value.
Keywords: buffalo; spermatozoa;
post thawing; semen quality
Author: H. C. Mahendra, D.
Samsudewa, Y. S. Ondho
Journal Code: jppeternakangg180004
